Product Description |
Recombinant Human SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Leu468. |
Product Background |
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs. |
Product Category |
Recombinant Protein |
Protein |
SG3/Secretogranin 3 |
Synonyms |
Secretogranin-3; Secretogranin III; SCG3; SGIII; UNQ2502/PRO5990 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe20-Leu468 |
Molecular Weight |
The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SG3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human SG3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. |
|
|
Human SG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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