Human Siglec-10 Protein (SIG-HM210)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. The affinity constant of 15.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met17-Thr546

Catalog: SIG-HM210

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Product Description Recombinant Human Siglec-10 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Met17-Thr546.
Product Category Recombinant Protein
Target Siglec-10
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
Gene/Protein ID Q96LC7-1
Species Human
Exact Sequence Met17-Thr546
Molecular Weight The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. The affinity constant of 15.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PB, 150mM NaCl, 25mM L-Arginine (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Siglec-10, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9μM as determined in SPR assay (Biacore T200).
Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA.
Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.3/7.7/7.4ng/ml determined by ELISA.
The purity of Human Siglec-10 is greater than 95% as determined by SEC-HPLC.
Human Siglec-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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