Human Siglec-10 Protein (SIG-HM210)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-10, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met17-Thr546

Catalog: SIG-HM210

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Product Description	Recombinant Human Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546.
Product Category	Recombinant Protein

Target	Siglec-10
Synonyms	SLG2; SIGLEC10; MGC126774; PRO940
Gene/Protein ID	Q96LC7-1
Species	Human
Exact Sequence	Met17-Thr546
Molecular Weight	The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Siglec-10, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 150mM NaCl, 25mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human Siglec-10, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9uM as determined in SPR assay (Biacore T200).
	Immobilized Human Siglec-10, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
	The purity of Human Siglec-10-is greater than 95% as determined by SEC-HPLC.
	Human Siglec-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.