Product Description |
Recombinant Human Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546. |
Product Background |
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
Product Category |
Recombinant Protein |
Target |
Siglec-10 |
Synonyms |
SLG2; SIGLEC10; MGC126774; PRO940 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met17-Thr546 |
Molecular Weight |
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PB, 150mM NaCl, 25mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human Siglec-10, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9uM as determined in SPR assay (Biacore T200). |
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Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). |
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Human Siglec-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Siglec-10-is greater than 95% as determined by SEC-HPLC. |
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