Human Siglec-10 Protein (SIG-HM210)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met17-Thr546
Catalog #SIG-HM210
Regular price $315.00
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Product Description
Recombinant Human Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546.
Product Background
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Product Category
Recombinant Protein
Target
Siglec-10
Synonyms
SLG2; SIGLEC10; MGC126774; PRO940
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met17-Thr546
Molecular Weight
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM PB, 150mM NaCl, 25mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Siglec-10, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9uM as determined in SPR assay (Biacore T200).
Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test).
Human Siglec-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Siglec-10-is greater than 95% as determined by SEC-HPLC.

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