Name: Human Siglec-10 Protein (SIG-HM210)
Brand: KactusBio
SKU: SIG-HM210-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Met17-Thr546

Catalog #SIG-HM210

Size

100UG

500UG

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Product Description	Recombinant Human Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546.
Product Background	Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Product Category	Recombinant Protein / Antibody discovery

Protein	Siglec-10
Synonyms	SLG2; SIGLEC10; MGC126774; PRO940
Accession	Q96LC7-1
Species	Human
Biotinylated	no
Amino Acid Range	Met17-Thr546
Molecular Weight	The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 150mM NaCl, 25mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Siglec-10, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CD52, mFc Tag with an affinity constant of 15.9uM as determined in SPR assay (Biacore T200).
	Immobilized Human Siglec-10, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test).
	Human Siglec-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Siglec-10-is greater than 95% as determined by SEC-HPLC.