Human Siglec-10 Protein (SIG-HM210)
Catalog: SIG-HM210
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Product Description | Recombinant Human Siglec-10 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Met17-Thr546. |
Product Category | Recombinant Protein |
Target | Siglec-10 |
Synonyms | SLG2; SIGLEC10; MGC126774; PRO940 |
Gene/Protein ID | Q96LC7-1 |
Species | Human |
Exact Sequence | Met17-Thr546 |
Molecular Weight | The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. The affinity constant of 15.9μM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in 20mM PB, 150mM NaCl, 25mM L-Arginine (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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