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Non-biotinylated Human Siglec-10 Protein (SIG-HM510) Summary
Product Description |
Recombinant Human Siglec-10 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Met17-Thr546. |
Product Background |
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Human Siglec-10 Protein (SIG-HM510) Specifications/Details
Protein |
Siglec-10 |
Synonyms |
SLG2; SIGLEC10; MGC126774; PRO940 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met17-Thr546 |
Molecular Weight |
The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1)-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in PBS, 0.5M Arginine (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. |
|
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Human Siglec-10 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95% |
|
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The purity of Human Siglec-10 is greater than 85% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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