Human Siglec-10 Protein (SIG-HM510)

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Met17-Thr546

Catalog: SIG-HM510

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Product Description	Recombinant Human Siglec-10 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met17-Thr546.
Product Background	Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Product Category	Recombinant Protein

Target	Siglec-10
Synonyms	SLG2; SIGLEC10; MGC126774; PRO940
Gene/Protein ID	Q96LC7-1
Species	Human
Exact Sequence	Met17-Thr546
Molecular Weight	The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
	The purity of Human Siglec-10 is greater than 95% as determined by SEC-HPLC.
	Human Siglec-10 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%