Human Siglec-10 Protein (SIG-HM510)

  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-10 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met17-Thr546

Catalog: SIG-HM510

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Product Description Recombinant Human Siglec-10 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal.It contains Met17-Thr546.
Product Category Recombinant Protein
Target Siglec-10
Synonyms SLG2; SIGLEC10; MGC126774; PRO940
Gene/Protein ID Q96LC7-1
Species Human
Exact Sequence Met17-Thr546
Molecular Weight The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Siglec-10 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-10 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA.
The purity of Human Siglec-10 is greater than 95% as determined by SEC-HPLC.
Human Siglec-10 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%

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