Human Siglec-10 Protein (SIG-HM510)
Human Siglec-10 Protein (SIG-HM510)
Human Siglec-10 Protein (SIG-HM510)
Human Siglec-10 Protein (SIG-HM510)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met17-Thr546
Catalog #SIG-HM510
/
Size

Order now, ships by:

Product Description
 Recombinant Human Siglec-10 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met17-Thr546.
Product Background
 Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Product Category
 Recombinant Protein
Target
 Siglec-10
Synonyms
 SLG2; SIGLEC10; MGC126774; PRO940
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met17-Thr546
Molecular Weight
 The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
Human Siglec-10 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%
The purity of Human Siglec-10 is greater than 95% as determined by SEC-HPLC.
Recently viewed