Non-biotinylated Human Siglec-10 Protein (SIG-HM510)

Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.

Human Siglec-10 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%

The purity of Human Siglec-10 is greater than 85% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Activity: Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
Amino Acid Range: Met17-Thr546

Catalog #SIG-HM510

Price

$346⁵⁰

Size

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Non-biotinylated Human Siglec-10 Protein (SIG-HM510) Summary

Product Description

Recombinant Human Siglec-10 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Product Background

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Non-biotinylated Human Siglec-10 Protein (SIG-HM510) Specifications/Details

Protein	Siglec-10
Synonyms	SLG2; SIGLEC10; MGC126774; PRO940
Accession	Q96LC7-1
Species	Human
Biotinylated	no
Amino Acid Range	Met17-Thr546
Molecular Weight	The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)-Avi
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in PBS, 0.5M Arginine (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Siglec-10, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
	Human Siglec-10 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%
	The purity of Human Siglec-10 is greater than 85% as determined by SEC-HPLC.