Human Siglec-10 (R119A) Protein (SIG-HM411)
Human Siglec-10 (R119A) Protein (SIG-HM411)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;
  • Activity: Immobilized Human Siglec-10 (R119A) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met17-Thr546 (R119A)
Catalog #SIG-HM411
Regular price $315.00
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Product Description
Recombinant Human Siglec-10 (R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.
Product Background
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Product Category
Recombinant Protein
Target
Siglec-10 (R119A)
Synonyms
SLG2; SIGLEC10; MGC126774; PRO940
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met17-Thr546 (R119A)
Molecular Weight
The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Siglec-10 (R119A) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-10 (R119A) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA.
Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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