Human Siglec-15/CD33L3 Protein (SIG-HM215)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Siglec-15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 37.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Lys24-Gly260

Catalog: SIG-HM215

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Product Description	Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys24-Gly260.
Product Background	Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Product Category	Recombinant Protein

Target	Siglec-15/CD33L3
Synonyms	CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Gene/Protein ID	Q6ZMC9-1
Species	Human
Exact Sequence	Lys24-Gly260
Molecular Weight	The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Siglec-15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 37.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Siglec-15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 37.3ng/ml determined by ELISA.
	Human Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.