Human Siglec-15/CD33L3 Protein (SIG-HM215)
Catalog: SIG-HM215
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| Product Description | Recombinant Human Siglec-15/CD33L3 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Lys24-Gly260. |
| Product Category | Recombinant Protein |
| Target | Siglec-15/CD33L3 |
| Synonyms | CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15 |
| Gene/Protein ID | Q6ZMC9-1 |
| Species | Human |
| Exact Sequence | Lys24-Gly260 |
| Molecular Weight | The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Siglec-15, hFc Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-Siglec-15 Ab., hFc Tag with the EC50 of 7.37ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Siglec-15, hFc Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-Siglec-15 Ab., hFc Tag with the EC50 of 7.37ng/ml determined by ELISA. |
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Human Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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