Human Siglec-15/CD33L3 Protein (SIG-HM215)
Catalog: SIG-HM215
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Product Description | Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys24-Gly260. |
Product Background |
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates. |
Product Category |
Recombinant Protein |
Target |
Siglec-15/CD33L3 |
Synonyms | CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15 |
Accession | |
Species |
Human |
Amino Acid Range |
Lys24-Gly260 |
Molecular Weight | The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human Siglec-15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 22.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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