Name: Non-biotinylated Human Siglec-15/CD33L3 Protein (SIG-HM415)
Brand: KactusBio
SKU: SIG-HM415-100UG
Price: 346.5 USD
Availability: InStock

Non-biotinylated Human Siglec-15/CD33L3 Protein (SIG-HM415)

Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).

Human Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Siglec-15 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Phe20-Thr263

Catalog #SIG-HM415

Size

100UG

500UG

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Non-biotinylated Human Siglec-15/CD33L3 Protein (SIG-HM415) Summary

Product Description	Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe20-Thr263.
Product Background	Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates..
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated Human Siglec-15/CD33L3 Protein (SIG-HM415) Specifications/Details

Protein	Siglec-15/CD33L3
Synonyms	CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Accession	Q6ZMC9
Species	Human
Biotinylated	no
Amino Acid Range	Phe20-Thr263
Molecular Weight	The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
	Human Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Siglec-15 is greater than 90% as determined by SEC-HPLC.