Human Siglec-15/CD33L3 Protein (SIG-HM415)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe20-Thr263

Catalog: SIG-HM415

$315.00
Size:

Order now get it on

Product Description Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe20-Thr263.

Product Background

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates..

Product Category

Recombinant Protein

Target

Siglec-15/CD33L3
Synonyms CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Accession

Species

Human

Amino Acid Range

Phe20-Thr263
Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
The purity of Human Siglec-15 is greater than 95% as determined by SEC-HPLC.
Human Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in