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Human Siglec-2/CD22 Protein, Ultra Low Endotoxin (SIG-HM122-UL) Summary
Product Description |
Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Arg687. |
Product Background |
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Human Siglec-2/CD22 Protein, Ultra Low Endotoxin (SIG-HM122-UL) Specifications/Details
Protein |
Siglec-2/CD22 |
Synonyms |
CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp20-Arg687 |
Molecular Weight |
The protein has a predicted MW of 76.2 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. |
|
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Human Siglec-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Siglec-2 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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