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Human Siglec-3/CD33 Protein, Ultra Low Endotoxin (SIG-HM203-UL) Summary
Product Description |
Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp18-His259. |
Product Background |
CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. CD33 is the smallest siglec member. It preferentially binds to α2-6- and α2-3-sialylated glycans and strongly binds to sialylated ligands on leukemic cell lines. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Human Siglec-3/CD33 Protein, Ultra Low Endotoxin (SIG-HM203-UL) Specifications/Details
Protein |
Siglec-3/CD33 |
Synonyms |
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp18-His259 |
Molecular Weight |
The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Siglec-3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Siglec-3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. |
|
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Human Siglec-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
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The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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