Human Siglec-4a/MAG Protein (MAG-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-4a, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly20-Pro516

Catalog: MAG-HM101

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Product Description Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Pro516.

Product Background

Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.

Product Category

Recombinant Protein

Target

Siglec-4a/MAG
Synonyms MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession

Species

Human

Amino Acid Range

Gly20-Pro516
Molecular Weight The protein has a predicted MW of 55.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Siglec-4a, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-4a, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
The purity of Human Siglec-4a is greater than 95% as determined by SEC-HPLC.
Human Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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