Human Siglec-4a/MAG Protein (SIG-HM24A)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-4a, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly20-Pro516

Catalog: SIG-HM24A

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Product Description Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly20-Pro516.

Product Background

Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.

Product Category

Recombinant Protein

Target

Siglec-4a/MAG
Synonyms MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession

Species

Human

Amino Acid Range

Gly20-Pro516
Molecular Weight The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Siglec-4a, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-4a, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA.
The purity of-Human Siglec-4a is greater than 95% as determined by SEC-HPLC.
Human Siglec-4a on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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