Human Siglec-4a/MAG Protein (SIG-HM24A)
Human Siglec-4a/MAG Protein (SIG-HM24A)
Human Siglec-4a/MAG Protein (SIG-HM24A)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly20-Pro516
Catalog #SIG-HM24A
Regular price $270.00
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Product Description
Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly20-Pro516.
Product Background
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
Product Category
Recombinant Protein
Target
Siglec-4a/MAG
Synonyms
MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly20-Pro516
Molecular Weight
The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Human Siglec-4a on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of-Human Siglec-4a is greater than 95% as determined by SEC-HPLC.

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