Name: Human Siglec-4a/MAG Protein (SIG-HM24A)
Brand: KactusBio
SKU: SIG-HM24A-100UG
Price: 270.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly20-Pro516

Catalog #SIG-HM24A

Size

100UG

500UG

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Product Description	Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly20-Pro516.
Product Background	Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
Product Category	Recombinant Protein

Protein	Siglec-4a/MAG
Synonyms	MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession	P20916
Species	Human
Biotinylated	no
Amino Acid Range	Gly20-Pro516
Molecular Weight	The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
	Human Siglec-4a on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of-Human Siglec-4a is greater than 95% as determined by SEC-HPLC.