Product Description |
Recombinant Human Siglec-5/CD170 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu17-Thr434. |
Product Background |
Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. |
Product Category |
Recombinant Protein |
Target |
Siglec-5/CD170 |
Synonyms |
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu17-Thr434 |
Molecular Weight |
The protein has a predicted MW of 73.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human Siglec-5 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
The purity of Human Siglec-5 is greater than 95% as determined by SEC-HPLC. |
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