Human Siglec-6/CD327 Protein (SIG-HM206)
Human Siglec-6/CD327 Protein (SIG-HM206)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Siglec-6, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln27-Val331
Catalog #SIG-HM206
Regular price $264.00
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Human Siglec-6/CD327 Protein (SIG-HM206) Summary

Product Description

Recombinant Human Siglec-6/CD327 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln27-Val331.

Product Background

Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Siglec-6/CD327 Protein (SIG-HM206) Specifications/Details

Protein
Siglec-6/CD327
Synonyms
CD327; CD33 antigen-like 1; CD33L1; CDw327; OB-BP1; Siglec-6; SIGLEC6; CD33L; OBBP1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln27-Val331
Molecular Weight
The protein has a predicted MW of 60.5 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Siglec-6, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
Product Tag
C-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Siglec-6, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test). Immobilized Human Siglec-6, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
Human Siglec-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Siglec-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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