- Express System: HEK293
- Product Tag: C-hFc (IgG1)
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human Siglec-6, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
- Amino Acid Range: Gln27-Val331
Human Siglec-6/CD327 Protein (SIG-HM206) Summary
Product Description |
Recombinant Human Siglec-6/CD327 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln27-Val331. |
Product Background |
Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Siglec-6/CD327 Protein (SIG-HM206) Specifications/Details
Protein |
Siglec-6/CD327 |
Synonyms |
CD327; CD33 antigen-like 1; CD33L1; CDw327; OB-BP1; Siglec-6; SIGLEC6; CD33L; OBBP1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln27-Val331 |
Molecular Weight |
The protein has a predicted MW of 60.5 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Siglec-6, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
Other Available Proteins:
Finding available proteins...
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