Product Description |
Recombinant Human Siglec-8 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met17-Ala363. |
Product Background |
Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Siglec-8 |
Synonyms |
CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met17-Ala363 |
Molecular Weight |
The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human Siglec-8 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
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The purity of-Human Siglec-8 is greater than 95% as determined by SEC-HPLC. |
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