Human Siglec-8 Protein (SIG-HM208) Summary
Product Description |
Recombinant Human Siglec-8 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met17-Ala363. |
Product Background |
Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Siglec-8 Protein (SIG-HM208) Specifications/Details
Protein |
Siglec-8 |
Synonyms |
CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met17-Ala363 |
Molecular Weight |
The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human Siglec-8 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
The purity of-Human Siglec-8 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
