Human Siglec-9 Protein (SIG-HM419)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Gln18-Gly348

Catalog: SIG-HM419

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Product Description Recombinant human Siglec-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Gly348.

Product Background

 Siglec-9 is a sialic-acid-binding lectin expressed predominantly on myeloid cells. Aberrant glycosylation occurs in essentially all types of cancers and results in increased sialylation. Thus, when the mucin MUC1 is expressed on cancer cells, it is decorated by multiple short, sialylated O-linked glycans (MUC1-ST).

Product Category

 Recombinant Protein

Target

 Siglec-9
Synonyms CD329; CDw329; FOAP-9; OBBP-Like; Siglec-9; SIGLEC9

Gene/Protein ID

 Q9Y336-1

Species

 Human

Exact Sequence

 Gln18-Gly348
Molecular Weight The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human Siglec-9 is greater than 95% as determined by SEC-HPLC.
Human Siglec-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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