Human Siglec-9 Protein, Ultra Low Endotoxin (SIG-HM419-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Gln18-Gly348
Catalog #SIG-HM419-UL
Regular price $381.00
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Product Description

Recombinant human Siglec-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Gly348.

Product Background

Siglec-9 is a sialic-acid-binding lectin expressed predominantly on myeloid cells. Aberrant glycosylation occurs in essentially all types of cancers and results in increased sialylation. Thus, when the mucin MUC1 is expressed on cancer cells, it is decorated by multiple short, sialylated O-linked glycans (MUC1-ST).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Siglec-9

Synonyms

CD329; CDw329; FOAP-9; OBBP-Like; Siglec-9; SIGLEC9

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln18-Gly348

Molecular Weight

The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 25mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 25mM MES, 150mM NaCl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Siglec-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Siglec-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Siglec-9 is greater than 90% as determined by SEC-HPLC. The purity of Human Siglec-9 is greater than 90% as determined by SEC-HPLC.

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support@kactusbio.us

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