Human SIRP alpha/CD172a Protein (SRP-HM172)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu31-Arg370

Catalog: SRP-HM172

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Product Description Recombinant Human SIRP alpha/CD172a Protein is expressed from Expi293 with His tag at the C-terminal.It contains Glu31-Arg370.
Product Category Recombinant Protein
Target SIRP alpha/CD172a
Synonyms CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID P78324-1
Species Human
Exact Sequence Glu31-Arg370
Molecular Weight The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human SIRP alpha, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200).
Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200).
Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA.
Serial dilutions of Anti-SIRP alpha Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, His Tag binding reactions.The half maximal inhibitory concentration (IC50) is 73.46ng/ml.
The purity of Human SIRP alpha is greater than 95% as determined by SEC-HPLC.
Human SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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