Human SIRP alpha/CD172a Protein (SRP-HM172)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu31-Arg370
Catalog #SRP-HM172
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Product Description
 Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg370.
Product Background
 Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category
 Recombinant Protein
Target
 SIRP alpha/CD172a
Synonyms
 CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu31-Arg370
Molecular Weight
 The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human SIRP alpha, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200).
Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200).
Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-SIRP alpha Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, His Tag binding reactions.The half maximal inhibitory concentration (IC50) is 73.46ng/ml.
Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SIRP alpha is greater than 95% as determined by SEC-HPLC.

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