Name: Human SIRP alpha/CD172a Protein (SRP-HM172)
Brand: KactusBio
SKU: SRP-HM172-100UG
Price: 297.0 USD
Availability: InStock

Human SIRP alpha/CD172a Protein (SRP-HM172)

Human SIRP alpha, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200).

Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200).

Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test).

Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Anti-SIRP alpha Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, His Tag binding reactions.The half maximal inhibitory concentration (IC50) is 73.46ng/ml.

The purity of Human SIRP alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu31-Arg370

Catalog #SRP-HM172

Size

100UG

500UG

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Product Description	Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg370.
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	SIRP alpha/CD172a
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA;
Accession	P78324-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu31-Arg370
Molecular Weight	The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human SIRP alpha, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200).
	Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200).
	Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test).
	Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-SIRP alpha Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, His Tag binding reactions.The half maximal inhibitory concentration (IC50) is 73.46ng/ml.
	The purity of Human SIRP alpha is greater than 95% as determined by SEC-HPLC.