Name: Human SIRP alpha/CD172a Protein (SRP-HM272)
Brand: KactusBio
SKU: SRP-HM272-100UG
Price: 297.0 USD
Availability: InStock

Human SIRP alpha/CD172a Protein (SRP-HM272)

Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human SIRP alpha, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).

Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test).

Immobilized Human SIRP alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 26.9ng/ml determined by ELISA.

Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SIRP alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu31-Arg370

Catalog #SRP-HM272

Size

100UG

500UG

Order now, ships by:

[{"id":42618396541150,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"SRP-HM272-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human SIRP alpha\/CD172a Protein (SRP-HM272) - 100UG","public_title":"100UG","options":["100UG"],"price":29700,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584263651550,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"SRP-HM272-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human SIRP alpha\/CD172a Protein (SRP-HM272) - 500UG","public_title":"500UG","options":["500UG"],"price":99000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu31-Arg370.
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	SIRP alpha/CD172a
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA;
Accession	P78324-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu31-Arg370
Molecular Weight	The protein has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human SIRP alpha, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test).
	Immobilized Human SIRP alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 26.9ng/ml determined by ELISA.
	Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SIRP alpha is greater than 95% as determined by SEC-HPLC.