Human SIRP alpha/CD172a Protein (SRP-HM272)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu31-Arg370

Catalog: SRP-HM272

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Product Description	Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu31-Arg370.
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein

Target	SIRP alpha/CD172a
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID	P78324-1
Species	Human
Exact Sequence	Glu31-Arg370
Molecular Weight	The protein has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human SIRP alpha, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test).
	Immobilized Human SIRP alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 26.9ng/ml determined by ELISA.
	The purity of SIRP alpha is greater than 95% as determined by SEC-HPLC.
	Human SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.