Name: Human SIRP alpha V2/CD172a Protein (SRP-HM1V2)
Brand: KactusBio
SKU: SRP-HM1V2-100UG
Price: 297.0 USD
Availability: InStock

SPR sensorgram showing multi-concentration binding curves for analyte-target interaction, used to determine kinetic affinity parameters.

Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).

Immobilized Human SIRP alpha V2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test).

Immobilized Human SIRP alpha V2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

Human SIRP alpha V2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.

The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP alpha V2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test). The affinity constant of 9.11nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu31-Arg369

Catalog #SRP-HM1V2

Size

100UG

500UG

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Product Description	Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg369.
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	SIRP alpha V2/CD172a
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA; SIRPalpha2
Accession	AAH26692.1
Species	Human
Biotinylated	no
Amino Acid Range	Glu31-Arg369
Molecular Weight	The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human SIRP alpha V2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test). The affinity constant of 9.11nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).
	Immobilized Human SIRP alpha V2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test).
	Immobilized Human SIRP alpha V2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
	Human SIRP alpha V2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.
	The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.