Human SIRP alpha V2/CD172a Protein (SRP-HM1V2)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP alpha V2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. The affinity constant of 9.11nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu31-Arg369

Catalog: SRP-HM1V2

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Product Description	Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg369.
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein

Target	SIRP alpha V2/CD172a
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID	AAH26692.1
Species	Human
Exact Sequence	Glu31-Arg369
Molecular Weight	The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human SIRP alpha V2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. The affinity constant of 9.11nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).
	Immobilized Human SIRP alpha V2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
	Immobilized Human SIRP alpha V2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
	Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.
	The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.
	Human SIRP alpha V2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.