Product Description |
Recombinant Human SIRP alpha V4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H). |
Product Background |
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. |
Product Category |
Recombinant Protein |
Target |
SIRP alpha V4 |
Synonyms |
CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu31-Arg370(E33G, L44S, T50S, T52I, R54H) |
Molecular Weight |
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SIRP alpha V4, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human SIRP alpha V4, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. |
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Human SIRP alpha V4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC. |
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