Human SIRP alpha V4 Protein (SRP-HM4V4)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP alpha V4, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)

Catalog: SRP-HM4V4

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Product Description	Recombinant Human SIRP alpha V4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H).
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein

Target	SIRP alpha V4
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID	P78324 variant 4
Species	Human
Exact Sequence	Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)
Molecular Weight	The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human SIRP alpha V4, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human SIRP alpha V4, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA.
	The purity of Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC.
	Human SIRP alpha V4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.