Human SIRP alpha V4 Protein (SRP-HM4V4)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SIRP alpha V4, His Tag at 1_g/ml (100_l/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 56.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)

Catalog: SRP-HM4V4

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Product Description Recombinant Human SIRP alpha V4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H).
Product Category Recombinant Protein
Target SIRP alpha V4
Synonyms CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3
Gene/Protein ID P78324 variant 4
Species Human
Exact Sequence Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)
Molecular Weight The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human SIRP alpha V4, His Tag at 1_g/ml (100_l/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 56.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human SIRP alpha V4, His Tag at 1_g/ml (100_l/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 56.8ng/ml determined by ELISA.
The purity of Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC.
Human SIRP alpha V4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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