Chromatogram showing the HPLC analysis with a peak at approximately 6.378 minutes. The y-axis represents the absorbance in mAU (milli-absorbance units), and the x-axis represents the time in minutes.
Human SIRP Gamma/CD172g Protein (SRP-HM40G)
Human SIRP Gamma/CD172g Protein (SRP-HM40G)
Human SIRP Gamma/CD172g Protein (SRP-HM40G)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu29-Pro360
Catalog #SRP-HM40G
Regular price $247.50
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Product Description

Recombinant Human SIRP Gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.

Product Background

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

SIRP Gamma/CD172g

Synonyms

CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2; SIRPγ; SIRP γ

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Glu29-Pro360

Molecular Weight

The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA. Immobilized Human SIRP Gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46ug/ml determined by ELISA.
Human SIRP Gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.. Human SIRP Gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%..
The purity of Human SIRP Gamma is greater than 95% as determined by SEC-HPLC. The purity of Human SIRP Gamma is greater than 95% as determined by SEC-HPLC.

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