Product Description |
Recombinant Human SLPI Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser26-Ala132. |
Product Background |
Elafin and SLPI (secretory leucocyte protease inhibitor) have multiple important roles both in normal homoeostasis and at sites of inflammation. These include antiprotease and antimicrobial activity as well as modulation of the response to LPS (lipopolysaccharide) stimulation. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. |
Product Category |
Recombinant Protein |
Target |
SLPI |
Synonyms |
ALP; BLPI; HUSI-1; MPI; WAP4; WFDC4; ALK1; HUSI; SLPI |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser26-Ala132 |
Molecular Weight |
The protein has a predicted MW of 38.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SLPI, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-SLPI Antibody, hFc Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 350mM NaCl (pH 7.4). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human SLPI, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-SLPI Antibody, hFc Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). |
|
|
Human SLPI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human SLPI is greater than 95% as determined by SEC-HPLC. |
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