Product Description |
Recombinant Human SMPD1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Pro628. |
Product Background |
Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme. |
Product Category |
Recombinant Protein |
Target |
SMPD1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Pro628 |
Molecular Weight |
The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
Baculovirus-Insect Cells |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human SMPD1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human SMPD1 is greater than 95% as determined by SEC-HPLC. |
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