Name: Human SMPD1 Protein (SMD-HB101)
Brand: KactusBio
SKU: SMD-HB101-100UG
Price: 412.5 USD
Availability: InStock

Express System: Baculovirus-Insect Cells(Sf9)
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is > 1500 pmol/min/ug.
Amino Acid Range: Met1-Pro628

Catalog #SMD-HB101

Size

100UG

500UG

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Human SMPD1 Protein (SMD-HB101) Summary

Product Description	Recombinant Human SMPD1 Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.It contains Met1-Pro628.
Product Background	Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human SMPD1 Protein (SMD-HB101) Specifications/Details

Protein	SMPD1
Synonyms	ASM;
Accession	NP_000534.3
Species	Human
Biotinylated	no
Amino Acid Range	Met1-Pro628
Molecular Weight	The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is > 1500 pmol/min/ug.
Product Tag	C-His
Expression System	Baculovirus-Insect Cells(Sf9)
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human SMPD1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human SMPD1 is greater than 95% as determined by SEC-HPLC.