Name: Human Sonic Hedgehog (Shh) Protein (SHH-HE001)
Brand: KactusBio
SKU: SHH-HE001-100UG
Price: 297.0 USD
Availability: InStock

Human Sonic Hedgehog (Shh) Protein (SHH-HE001)

Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50-for this effect is 0.1-0.4 µg/mL.

The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Cys24-Gly197

Catalog #SHH-HE001

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human Sonic Hedgehog (Shh) Protein (SHH-HE001) Summary

Product Description	Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197.
Product Background	Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human Sonic Hedgehog (Shh) Protein (SHH-HE001) Specifications/Details

Protein	Sonic Hedgehog (Shh)
Synonyms	SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS
Accession	Q15465
Species	Human
Biotinylated	no
Amino Acid Range	Cys24-Gly197
Molecular Weight	The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50-for this effect is 0.1-0.4 µg/mL.
	The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.