Human Sonic Hedgehog (Shh) Protein (SHH-HE001)
Catalog: SHH-HE001
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| Product Description | Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197. |
Product Background |
Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages. |
Product Category |
Recombinant Protein |
Target |
Sonic Hedgehog (Shh) |
Gene/Protein ID |
Q15465 |
Species |
Human |
Exact Sequence |
Cys24-Gly197 |
| Molecular Weight | The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Tris-Bis PAGE result. |
Product Tag |
No Tag |
Express System |
E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC. |
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Human Sonic Hedgehog (Shh) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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