Product Description |
Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197. |
Product Background |
Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages. |
Product Category |
Recombinant Protein |
Target |
Sonic Hedgehog (Shh) |
Synonyms |
SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys24-Gly197 |
Molecular Weight |
The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.05EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC. |
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