Regular price
$375.00
Order by 4pm ET for delivery on:
Human SOST/Sclerostin Protein (SOT-HM201) Summary
Product Description |
Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Gln24-Tyr213. |
Product Background |
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human SOST/Sclerostin Protein (SOT-HM201) Specifications/Details
Protein |
SOST/Sclerostin |
Synonyms |
sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Tyr213 |
Molecular Weight |
The protein has a predicted MW of 47.05 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SOST, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human SOST, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
|
|
Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
