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Human SOST/Sclerostin Protein, Ultra Low Endotoxin (SOT-HM201-UL) Summary
Product Description |
Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Gln24-Tyr213. |
Product Background |
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human SOST/Sclerostin Protein, Ultra Low Endotoxin (SOT-HM201-UL) Specifications/Details
Protein |
SOST/Sclerostin |
Synonyms |
sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Tyr213 |
Molecular Weight |
The protein has a predicted MW of 47.05 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SOST, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human SOST, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
|
|
Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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