Product Description |
Recombinant Human SP17 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Lys151. |
Product Background |
Sperm protein 17 (Sp17) is a highly conserved mammalian protein characterized in rabbit, mouse, monkey, baboon, macaque, human testis and spermatozoa. mRNA encoding Sp17 has been detected in a range of murine and human somatic tissues. It was also recognized in two myeloma cell lines and in neoplastic cells from patients with multiple myeloma and ovarian carcinoma. |
Product Category |
Recombinant Protein |
Target |
SP17 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Lys151 |
Molecular Weight |
The protein has a predicted MW of 18.50 kDa. The protein migrates to 20-28 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCL (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human SP17 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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