Product Description |
Recombinant Human SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303. |
Product Background |
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed. |
Product Category |
Recombinant Protein |
Target |
SPARC |
Synonyms |
SPARC; BM-40; ON |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala18-Ile303 |
Molecular Weight |
The protein has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. |
|
|
Human SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human SPARC is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
