Human SPARC Protein (SPA-HM101)
Human SPARC Protein (SPA-HM101)
Human SPARC Protein (SPA-HM101)
Human SPARC Protein (SPA-HM101)
Human SPARC Protein (SPA-HM101)
Human SPARC Protein (SPA-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala18-Ile303
Catalog #SPA-HM101
/
Size

Order now, ships by:

Product Description
 Recombinant Human SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
Product Background
 Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Product Category
 Recombinant Protein
Target
 SPARC
Synonyms
 SPARC; BM-40; ON
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala18-Ile303
Molecular Weight
 The protein has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA.
Human SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SPARC is greater than 95% as determined by SEC-HPLC.
Recently viewed