Name: Human SPARC Protein (SPA-HM101)
Brand: KactusBio
SKU: SPA-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA.
Amino Acid Range: Ala18-Ile303

Catalog #SPA-HM101

Size

100UG

500UG

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Human SPARC Protein (SPA-HM101) Summary

Product Description	Recombinant Human SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
Product Background	Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human SPARC Protein (SPA-HM101) Specifications/Details

Protein	SPARC
Synonyms	SPARC; BM-40; ON
Accession	P09486
Species	Human
Biotinylated	no
Amino Acid Range	Ala18-Ile303
Molecular Weight	The protein has a predicted MW of 33.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human SPARC, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA.
	Human SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human SPARC is greater than 95% as determined by SEC-HPLC.