Human SPP1/OPN Protein (SPP-HM101)
Catalog: SPP-HM101
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| Product Description | Recombinant Human SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Asn300. |
| Product Category | Recombinant Protein |
| Target | SPP1/OPN |
| Synonyms | Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI |
| Gene/Protein ID | NP_000573.1 |
| Species | Human |
| Exact Sequence | Ile17-Asn300 |
| Molecular Weight | The protein has a predicted MW of 34.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
| Product Tag | C-His |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human SPP1 is greater than 95% as determined by SEC-HPLC. |
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Human SPP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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