Human Tau-441/2N4R Pre-formed Fibrils Protein (TAU-HE01F)

Express System: E.coli
Product Tag: No Tag
Activity: Immobilized Human Tau-441 Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Etalanetug, hFc Tag with the EC50 of 0.67ng/ml determined by ELISA.
Amino Acid Range: Met1-Leu441

Catalog #TAU-HE01F

Price

$450⁰⁰

Size

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Human Tau-441/2N4R Pre-formed Fibrils Protein (TAU-HE01F) Summary

Product Description

Recombinant Human Tau-441/2N4R Pre-formed Fibrils Protein is expressed from E.coli without tag.It contains Met1-Leu441.

Product Background

Tau is an intrinsically disordered protein that stabilizes microtubules (MTs) to promote axonal transport. In Alzheimer's disease (AD), this highly soluble protein aggregates into intracellular neurofibrillary tangles, whose spreading in the brain is the basis of current neuropathological staging of AD. Tau amyloid formation also occurs in other neurodegenerative diseases such as corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP).Although the precise molecular mechanism of tau aggregation is still largely unknown, many structures of aggregated tau have now been determined. Early transmission electron microscopy (TEM) images of paired helical filaments (PHFs) and straight filaments of AD tau showed a fuzzy exterior, which can be removed by pronase to reveal an ordered core.

Human Tau-441/2N4R Pre-formed Fibrils Protein (TAU-HE01F) Specifications/Details

Protein	Tau
Synonyms	PHF-tau; MAPT; MTBT1; TAU; Tau-F; Tau-4; 2N4R; DDPAC; FTDP-17; MSTD;Tau4; FTDP17
Accession	P10636-8
Species	Human
Biotinylated	no
Amino Acid Range	Met1-Leu441
Molecular Weight	The Tau-441/2N4R monomer protein has a predicted MW of 45.84 kDa.
Activity	Immobilized Human Tau-441 Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Etalanetug, hFc Tag with the EC50 of 0.67ng/ml determined by ELISA.
Product Tag	No Tag
Fluorophore	Unconjugated
Expression System	E.coli
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 25 mM HEPES, 250 mM NaCI,10% Glycerol, 1 mM TCEP (pH 7.2).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C. Do not store Pre-formed Fibrils at 4°C.； Recommend to aliquot the Pre-formed Fibrils protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Tau-441 Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Etalanetug, hFc Tag with the EC50 of 0.67ng/ml determined by ELISA.
	Negative-stain transmission electron microscopy (TEM) images of Human Tau-441 Pre-formed Fibrils, No Tag. Distinct fibrillar structures are visible in TEM micrographs of this product.