Human TDGF1/Cripto Protein (TDG-HM101)

Human ACVR1B, hFc Tag captured on CM5 Chip via Protein A can bind Human TDGF1, His Tag with an affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).

Human TDGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human ACVR1B, hFc Tag on ProA-Biosensor can bind Human TDGF1, His Tag with an affinity constant of 0.13 µM as determined in BLI assay .

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu31-Thr172

Catalog #TDG-HM101

Price

$297⁰⁰

Size

Order by 4pm ET for delivery on:

Human TDGF1/Cripto Protein (TDG-HM101) Summary

Product Description

Recombinant Human TDGF1/Cripto Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu31-Thr172.

Product Background

TDGF1 (CRIPTO) is a member of the epidermal growth factor-Cripto-1/FRL-1/Cryptic (EGF/CFC) gene family and an obligate co-receptor involved in NODAL signaling, a developmental program implicated in midline, forebrain, and left-right axis development in model organisms. Cripto-1 is enriched in a subpopulation of embryonal, melanoma, prostate, and pancreatic cancer cells that possess stem-like characteristics. Therefore, Cripto-1 may play a role during developmental EMT, and it may also be involved in the reprogramming of differentiated tumor cells into cancer stem cells through the induction of an EMT program.

Human TDGF1/Cripto Protein (TDG-HM101) Specifications/Details

Protein	TDGF1/Cripto
Synonyms	TDGF1; Cripto; CRGF; CRIPTO; CR
Accession	P13385
Species	Human
Biotinylated	no
Amino Acid Range	Leu31-Thr172
Molecular Weight	The protein has a predicted MW of 16.92 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human ACVR1B, hFc Tag captured on CM5 Chip via Protein A can bind Human TDGF1, His Tag with an affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).
	Human TDGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human ACVR1B, hFc Tag on ProA-Biosensor can bind Human TDGF1, His Tag with an affinity constant of 0.13 µM as determined in BLI assay .