Name: Human TDGF1/Cripto Protein (TDG-HM101)
Brand: KactusBio
SKU: TDG-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu31-Thr172

Catalog #TDG-HM101

Size

100UG

500UG

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Human TDGF1/Cripto Protein (TDG-HM101) Summary

Product Description	Recombinant Human TDGF1/Cripto Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu31-Thr172.
Product Background	TDGF1 (CRIPTO) is a member of the epidermal growth factor-Cripto-1/FRL-1/Cryptic (EGF/CFC) gene family and an obligate co-receptor involved in NODAL signaling, a developmental program implicated in midline, forebrain, and left-right axis development in model organisms. Cripto-1 is enriched in a subpopulation of embryonal, melanoma, prostate, and pancreatic cancer cells that possess stem-like characteristics. Therefore, Cripto-1 may play a role during developmental EMT, and it may also be involved in the reprogramming of differentiated tumor cells into cancer stem cells through the induction of an EMT program.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human TDGF1/Cripto Protein (TDG-HM101) Specifications/Details

Protein	TDGF1/Cripto
Synonyms	TDGF1; Cripto; CRGF; CRIPTO; CR
Accession	P13385
Species	Human
Biotinylated	no
Amino Acid Range	Leu31-Thr172
Molecular Weight	The protein has a predicted MW of 16.92 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human ACVR1B, hFc Tag captured on CM5 Chip via Protein A can bind Human TDGF1, His Tag with an affinity constant of 0.16 uM as determined in SPR assay (Biacore T200).
	Human TDGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human ACVR1B, hFc Tag on ProA-Biosensor can bind Human TDGF1, His Tag with an affinity constant of 0.13 µM as determined in BLI assay .

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