Product Description |
Recombinant Human Tenascin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly23-Ser621. |
Product Background |
Tenascin-C (TNC) is a hexameric, multimodular extracellular matrix protein with several molecular forms that are created through alternative splicing and protein modifications. It is highly conserved amongst vertebrates, and molecular phylogeny indicates that it evolved before fibronectin. Tenascin-C has many extracellular binding partners, including matrix components, soluble factors and pathogens; it also influences cell phenotype directly through interactions with cell surface receptors. |
Product Category |
Recombinant Protein |
Target |
Tenascin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly23-Ser621 |
Molecular Weight |
The protein has a predicted MW of 65.27 kDa. Due to glycosylation, the protein migrates to 66-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human Tenascin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Tenascin is greater than 95% as determined by SEC-HPLC. |
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