Product Description |
Recombinant Human TFPI-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Lys213. |
Product Background |
Tissue factor pathway inhibitor-2 (TFPI-2) has previously been characterized as an endogenous anticoagulant. TFPI-2 is expressed in the vast majority of cells, mainly secreted into the extracellular matrix. Moreover, in sputum from cystic fibrosis patients TFPI-2 C-terminal fragments are generated and found associated with immunoglobulins. Together our data describe a previously unknown host defense mechanism and therapeutic importance of TFPI-2 against invading Gram-negative bacterial pathogens. |
Product Category |
Recombinant Protein |
Target |
TFPI-2 |
Synonyms |
TFPI-2; PP5; TFPI2; REF1; TFPI-2REF1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp23-Lys213. |
Molecular Weight |
The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human TFPI-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 5 nM. |
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