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Non-biotinylated Human TFPI Protein (TFP-HM401) Summary
Product Description |
Recombinant Human TFPI Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp29-Lys282. |
Product Background |
Oestrogens influence the pathophysiology and development of hormone-sensitive cancers, such as breast cancer. Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation pathway and has recently been associated with breast cancer cell development. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human TFPI Protein (TFP-HM401) Specifications/Details
Protein |
TFPI |
Synonyms |
LACI; TFPI1; EPI; TFI; convertin; TFPI |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp29-Lys282 |
Molecular Weight |
The protein has a predicted MW of 32.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 0.75 nM. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Human TFPI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human TFPI is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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