Product Description |
Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val40-Ala89. |
Product Background |
Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice. |
Product Category |
Recombinant Protein |
Target |
TGF-alpha |
Synonyms |
ETGF; TGF-alpha; TGF type 1; TGFA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val40-Ala89 |
Molecular Weight |
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test). |
|
|
Human TGF-alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC. |
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