Human TGF-alpha Protein (TGF-HM201)

  • Express System: Expi293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val40-Ala89

Catalog: TGF-HM201

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Product Description Recombinant Human TGF-alpha Protein is expressed from Expi293 with hFc tag at the N-terminal.It contains Val40-Ala89.
Product Category Recombinant Protein
Target TGF-alpha
Synonyms ETGF; TGF-alpha; TGF type 1; TGFA
Gene/Protein ID P01135-1
Species Human
Exact Sequence Val40-Ala89
Molecular Weight The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay (Biacore T200).
Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.
Human TGF-alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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