Name: Human TGF-alpha Protein (TGF-HM201)
Brand: KactusBio
SKU: TGF-HM201-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Val40-Ala89

Catalog #TGF-HM201

Size

100UG

500UG

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Product Description	Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val40-Ala89.
Product Background	Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice.
Product Category	Recombinant Protein

Protein	TGF-alpha
Synonyms	ETGF; TGF-alpha; TGF type 1; TGFA
Accession	P01135-1
Species	Human
Biotinylated	no
Amino Acid Range	Val40-Ala89
Molecular Weight	The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
	Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).
	Human TGF-alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.