Human TGF-beta RII/TGFBR2 Protein (TGF-HM5R2)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Mature TGF beta 1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). The affinity constant of 2.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ile24-Asp159

Catalog: TGF-HM5R2

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Product Description Recombinant Human TGF-beta RII/TGFBR2 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ile24-Asp159.
Product Category Recombinant Protein
Target TGF-beta RII/TGFBR2
Synonyms TGFR2; TGFBR2; TbetaR-II; TGFβR2; TbetaR-II; TGFβR2; AAT3; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; TAAD2
Gene/Protein ID P37173-1
Species Human
Exact Sequence Ile24-Asp159
Molecular Weight The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Mature TGF beta 1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). The affinity constant of 2.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind Human Latent TGF beta 2, His Tag with an affinity constant of 2.21 uM as determined in SPR assay (Biacore T200).
Immobilized Human Mature TGF beta 1, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).
The purity of Human TGF-beta RII is greater than 95% as determined by SEC-HPLC.
Human TGF-beta RII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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