Product Description |
Recombinant Human TIE2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Leu748. |
Product Background |
Angiopoietin-1 (Ang-1) is the primary agonist for Tie2 tyrosine kinase receptor (Tie2), and the effect of Ang-1-Tie2 signalling is context-dependent. Deficiency in either Ang-1 or Tie2 protein leads to severe microvascular defects and subsequent embryonic lethality in murine model. Tie2 receptors are expressed in several cell types, including endothelial cells, smooth muscle cells, fibroblasts, epithelial cells, monocytes, neutrophils, eosinophils and glial cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
TIE2 |
Synonyms |
Tie-2; Angiopoietin-1 receptor; TEK; VMCM; VMCM1; CD202b |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala23-Leu748 |
Molecular Weight |
The protein has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 2.38 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 uM as determined in SPR assay (Biacore T200). |
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Human TIE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TIE2 is greater than 95% as determined by SEC-HPLC. |
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