Name: Human TIE2 Protein (TIE-HM201)
Brand: KactusBio
SKU: TIE-HM201-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 2.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala23-Leu748

Catalog #TIE-HM201

Size

100UG

500UG

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Human TIE2 Protein (TIE-HM201) Summary

Product Description	Recombinant Human TIE2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Leu748.
Product Background	Angiopoietin-1 (Ang-1) is the primary agonist for Tie2 tyrosine kinase receptor (Tie2), and the effect of Ang-1-Tie2 signalling is context-dependent. Deficiency in either Ang-1 or Tie2 protein leads to severe microvascular defects and subsequent embryonic lethality in murine model. Tie2 receptors are expressed in several cell types, including endothelial cells, smooth muscle cells, fibroblasts, epithelial cells, monocytes, neutrophils, eosinophils and glial cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human TIE2 Protein (TIE-HM201) Specifications/Details

Protein	TIE2
Synonyms	Tie-2; Angiopoietin-1 receptor; TEK; VMCM; VMCM1; CD202b
Accession	AAA61139.1
Species	Human
Biotinylated	no
Amino Acid Range	Ala23-Leu748
Molecular Weight	The protein has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 2.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 uM as determined in SPR assay (Biacore T200).
	Human TIE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TIE2 is greater than 95% as determined by SEC-HPLC.