Human TIGIT Protein (TIG-HM210)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Met22-Pro141

Catalog: TIG-HM210

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Product Description	Recombinant Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met22-Pro141.
Product Background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Product Category	Recombinant Protein

Target	TIGIT
Synonyms	TIGIT; VSIG9; VSTM3; WUCAM
Accession	Q495A1-1
Species	Human
Amino Acid Range	Met22-Pro141
Molecular Weight	The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
	Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test).
	Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
	The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
	Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.