Human TIGIT Protein (TIG-HM210)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met22-Pro141
Catalog #TIG-HM210
Regular price $330.00
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Product Description

Recombinant Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met22-Pro141.

Product Background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

TIGIT

Synonyms

TIGIT; VSIG9; VSTM3; WUCAM

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Met22-Pro141

Molecular Weight

The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC. The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.

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