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Human TIGIT Protein, Ultra Low Endotoxin (TIG-HM210-UL) Summary
Product Description |
Recombinant Human TIGIT Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Met22-Pro141. |
Product Background |
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human TIGIT Protein, Ultra Low Endotoxin (TIG-HM210-UL) Specifications/Details
Protein |
TIGIT |
Synonyms |
TIGIT; VSIG9; VSTM3; WUCAM |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met22-Pro141 |
Molecular Weight |
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. |
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Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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