Human TIMP-2 Protein (TIM-HM1P2)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Cys27-Pro220

Catalog: TIM-HM1P2

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Product Description	Recombinant Human TIMP-2 Protein Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys27-Pro220.
Product Background	Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.
Product Category	Recombinant Protein

Target	TIMP-2
Synonyms	CSC-21K; TIMP2; DDC8; TIMP-2
Gene/Protein ID	P16035
Species	Human
Exact Sequence	Cys27-Pro220
Molecular Weight	The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
	The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC.
	Human TIMP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.