Human TIMP-2 Protein (TIM-HM1P2)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Human TIMP-2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Cys27-Pro220

Catalog: TIM-HM1P2

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Product Description Recombinant Human TIMP-2 Protein Protein is expressed from Expi293 with His tag at the C-terminal.It contains Cys27-Pro220.
Product Category Recombinant Protein
Target TIMP-2
Synonyms CSC-21K; TIMP2; DDC8; TIMP-2
Gene/Protein ID P16035
Species Human
Exact Sequence Cys27-Pro220
Molecular Weight The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Human TIMP-2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Human TIMP-2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA.
The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC.
Human TIMP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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