Human TIMP-2 Protein (TIM-HM1P2)

Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

Human TIMP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Amino Acid Range: Cys27-Pro220

Catalog #TIM-HM1P2

Price

$264⁰⁰

Size

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Human TIMP-2 Protein (TIM-HM1P2) Summary

Product Description

Recombinant Human TIMP-2 Protein Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys27-Pro220.

Product Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.

Human TIMP-2 Protein (TIM-HM1P2) Specifications/Details

Protein	TIMP-2
Synonyms	CSC-21K; TIMP2; DDC8; TIMP-2;CSC21K
Accession	P16035
Species	Human
Biotinylated	no
Amino Acid Range	Cys27-Pro220
Molecular Weight	The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
	Human TIMP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC.