Human TIMP-2 Protein (TIM-HM1P2)
Human TIMP-2 Protein (TIM-HM1P2)
Human TIMP-2 Protein (TIM-HM1P2)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Cys27-Pro220
Catalog #TIM-HM1P2
Regular price $240.00
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Product Description

Recombinant Human TIMP-2 Protein Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys27-Pro220.

Product Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.

Product Category

Recombinant Protein / Antibody discovery

Protein

TIMP-2

Synonyms

CSC-21K; TIMP2; DDC8; TIMP-2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Cys27-Pro220

Molecular Weight

The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human TIMP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TIMP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC. The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC.

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