Product Description |
Recombinant Human TIMP-2 Protein Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys27-Pro220. |
Product Background |
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities. |
Product Category |
Recombinant Protein |
Target |
TIMP-2 |
Synonyms |
CSC-21K; TIMP2; DDC8; TIMP-2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys27-Pro220 |
Molecular Weight |
The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Human TIMP-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. |
|
|
Human TIMP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC. |
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