Human TLR3 Protein (TLR-HM103)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser23-Glu703

Catalog: TLR-HM103

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Product Description Recombinant Human TLR3 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ser23-Glu703.
Product Category Recombinant Protein
Target TLR3
Synonyms TLR3; CD283; IIAE2
Gene/Protein ID Q6PCD4
Species Human
Exact Sequence Ser23-Glu703
Molecular Weight The protein has a predicted MW of 78.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.
The purity of Human TLR3 is greater than 95% as determined by SEC-HPLC.
Human TLR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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