Human TLR3 Protein (TLR-HM103)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TLR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser23-Glu703

Catalog: TLR-HM103

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Product Description	Recombinant Human TLR3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser23-Glu703.
Product Background	TLR3 is expressed in the central nervous system (CNS), where it is required to control HSV-1, which spreads from the epithelium to the CNS via cranial nerves. TLR3 is also expressed in epithelial and dendritic cells, which apparently use TLR3-independent pathways to prevent further dissemination of HSV-1 and to provide resistance to other pathogens in TLR3-deficient patients. Human TLR3 appears to be redundant in host defense to most microbes but is vital for natural immunity to HSV-1 in the CNS, which suggests that neurotropic viruses have contributed to the evolutionary maintenance of TLR3.
Product Category	Recombinant Protein

Target	TLR3
Synonyms	TLR3; CD283; IIAE2
Gene/Protein ID	Q6PCD4
Species	Human
Exact Sequence	Ser23-Glu703
Molecular Weight	The protein has a predicted MW of 78.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TLR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human TLR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
	The purity of Human TLR3 is greater than 95% as determined by SEC-HPLC.
	Human TLR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.