Human TNF alpha Protein (active trimer) (TNF-HM00A)

Immobilized Human TNF alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag (Cat. TNF-HM2R1) with the EC50 of 2.7ng/ml determined by ELISA.

Immobilized Human TNF alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with the EC50 of 15.1ng/ml determined by ELISA.

Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL.

The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TNF alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag (Cat. TNF-HM2R1) with the EC50 of 2.7ng/ml determined by ELISA.
Amino Acid Range: Val77-Leu233

Catalog #TNF-HM00A

Price

$120⁰⁰

Size

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Human TNF alpha Protein (active trimer) (TNF-HM00A) Summary

Product Description

Recombinant Human TNF alpha Protein is expressed from HEK293 without tag.It contains Val77-Leu233.

Product Background

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Human TNF alpha Protein (active trimer) (TNF-HM00A) Specifications/Details

Protein	TNF alpha
Synonyms	APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha;TNF alpha
Accession	P01375
Species	Human
Biotinylated	no
Amino Acid Range	Val77-Leu233
Molecular Weight	The protein has a predicted MW of 17.35 kDa. Due to glycosylation, the protein migrates to 18-22 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNF alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag (Cat. TNF-HM2R1) with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag	No Tag
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human TNF alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag (Cat. TNF-HM2R1) with the EC50 of 2.7ng/ml determined by ELISA.
	Immobilized Human TNF alpha, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with the EC50 of 15.1ng/ml determined by ELISA.
	Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL.
	The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC.