Human TNFR1/CD120a/TNFRSF1A Protein (TNF-HM1R1)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNFR1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu30-Thr211

Catalog: TNF-HM1R1

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Product Description Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.

Product Background

Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.

Product Category

Recombinant Protein

Target

TNFR1/CD120a/TNFRSF1A
Synonyms TNF-R1; TNF-RI; TNFR-I; TNFR1; CD120a; TNFRSF1A; FPF; TBP1; TNF-R; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNF-R-I; TNFR1-d2
Accession

Species

Human

Amino Acid Range

Leu30-Thr211
Molecular Weight The protein has a predicted MW of 23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TNFR1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TNFR1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.5ng/ml determined by ELISA.
The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC.
Human TNFR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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