Product Description |
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu30-Thr211. |
Product Background |
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling. |
Product Category |
Recombinant Protein |
Target |
TNFR1/CD120a/TNFRSF1A |
Synonyms |
TNF-R1; TNF-RI; TNFR-I; TNFR1; CD120a; TNFRSF1A; FPF; TBP1; TNF-R; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNF-R-I; TNFR1-d2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu30-Thr211 |
Molecular Weight |
The protein has a predicted MW of 48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TNF alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human TNF alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
|
|
Human TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
