Human TNFR1/CD120a/TNFRSF1A Protein (TNF-HM2R1)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human TNF alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 22.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu30-Thr211

Catalog: TNF-HM2R1

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Product Description Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Leu30-Thr211.
Product Category Recombinant Protein
Target TNFR1/CD120a/TNFRSF1A
Synonyms TNF-R1; TNF-RI; TNFR-I; TNFR1; CD120a; TNFRSF1A; FPF; TBP1; TNF-R; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNF-R-I; TNFR1-d2
Gene/Protein ID P19438-1
Species Human
Exact Sequence Leu30-Thr211
Molecular Weight The protein has a predicted MW of 48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TNF alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 22.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TNF alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 22.1ng/ml determined by ELISA.
The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC.
Human TNFR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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