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Human TNFR1/CD120a/TNFRSF1A Protein, Ultra Low Endotoxin (TNF-HM1R1-UL) Summary
Product Description |
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211. |
Product Background |
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human TNFR1/CD120a/TNFRSF1A Protein, Ultra Low Endotoxin (TNF-HM1R1-UL) Specifications/Details
Protein |
TNFR1/CD120a/TNFRSF1A |
Synonyms |
TNF-R1; TNF-RI; TNFR-I; TNFR1; CD120a; TNFRSF1A; FPF; TBP1; TNF-R; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNF-R-I; TNFR1-d2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu30-Thr211 |
Molecular Weight |
The protein has a predicted MW of 23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TNFR1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.5ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human TNFR1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.5ng/ml determined by ELISA. |
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Human TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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