Product Description |
Recombinant Human TNFR2/CD120b/TNFRSF1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257. |
Product Background |
Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
Product Category |
Recombinant Protein |
Target |
TNFR2/CD120b/TNFRSF1B |
Synonyms |
CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu23-Asp257 |
Molecular Weight |
The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TNFR2, His Tag at 5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human TNFR2, His Tag at 5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). |
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Immobilized Human TNFR2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. |
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Human TNFR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC. |
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