Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 61.0ng/ml determined by ELISA. The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu23-Asp257

Catalog: TNF-HM3R2

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Product Description Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu23-Asp257.

Product Background

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.

Product Category

Recombinant Protein

Target

TNFR2/CD120b/TNFRSF1B
Synonyms CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B

Gene/Protein ID

P20333

Species

Human

Exact Sequence

Leu23-Asp257
Molecular Weight The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 61.0ng/ml determined by ELISA. The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 61.0ng/ml determined by ELISA.
Immobilized Human TNFR2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).
The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC.
Human TNFR2-on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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