Name: Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2)
Brand: KactusBio
SKU: TNF-HM3R2-100UG
Price: 264.0 USD
Availability: InStock

Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2)

Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).

Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).

Immobilized Human TNFR2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.

Human TNFR2-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Human TNFR2 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu23-Asp257

Catalog #TNF-HM3R2

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2) Summary

Product Description	Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu23-Asp257.
Product Background	Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human TNFR2/CD120b/TNFRSF1B Protein (TNF-HM3R2) Specifications/Details

Protein	TNFR2/CD120b/TNFRSF1B
Synonyms	CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B
Accession	P20333
Species	Human
Biotinylated	no
Amino Acid Range	Leu23-Asp257
Molecular Weight	The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Product Tag	C-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).
	Immobilized Human TNFR2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
	Human TNFR2-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human TNFR2 is greater than 90% as determined by SEC-HPLC.