Product Description |
Recombinant Human TNFRSF11B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Leu401. |
Product Background |
Osteoprotegerin (OPG) is a key regulator in bone metabolism, that also has effect in vascular system. Studies suggest that osteoprotegerin is a critical arterial calcification inhibitor, and is released by endothelial cells as a protective mechanism for their survival in certain pathological conditions, such as diabetes mellitus, chronic kidney disease, and other metabolic disorders. |
Product Category |
Recombinant Protein |
Target |
TNFRSF11B |
Synonyms |
Osteoprotegerin; Ocif; Opg; PDB5; TR1; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu22-Leu401 |
Molecular Weight |
The protein has a predicted MW of 44.71 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.2ng/ml determined by ELISA (QC Test). |
|
|
Human TNFRSF11B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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