Human TNFRSF11B Protein (TNF-HM11B)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu22-Leu401

Catalog: TNF-HM11B

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Product Description	Recombinant Human TNFRSF11B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Leu401.
Product Background	Osteoprotegerin (OPG) is a key regulator in bone metabolism, that also has effect in vascular system. Studies suggest that osteoprotegerin is a critical arterial calcification inhibitor, and is released by endothelial cells as a protective mechanism for their survival in certain pathological conditions, such as diabetes mellitus, chronic kidney disease, and other metabolic disorders.
Product Category	Recombinant Protein

Target	TNFRSF11B
Accession	O00300
Species	Human
Amino Acid Range	Glu22-Leu401
Molecular Weight	The protein has a predicted MW of 44.71 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
	Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test).
	Human TNFRSF11B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.