Human TNFRSF12A/TWEAKR Protein (TNF-HM20A)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human TNFRSF12A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu28-Per80

Catalog: TNF-HM20A

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Product Description Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Glu28-Per80.
Product Category Recombinant Protein
Target TNFRSF12A/TWEAKR
Synonyms FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR
Gene/Protein ID Q9NP84-1
Species Human
Exact Sequence Glu28-Per80
Molecular Weight The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TNFRSF12A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFRSF12A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).
Human TNFRSF12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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