Human TNFRSF12A/TWEAKR Protein (TNF-HM20A)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu28-Per80
Catalog #TNF-HM20A
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Product Description
 Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Glu28-Per80.
Product Background
 Thyroid cancer (TC) is the most well-known endocrine neoplasia as well as a common malignant tumor in the head and neck.TNFRSF12A expression may be a potential useful prognostic molecular biomarker of bad survival in thyroid cancer, in addition, PPAR signaling pathway, insulin signaling pathway, mTOR signaling pathway may be the key pathway controlled by TNFRSF12A in thyroid cancer. Further experimental ought to be performed to demonstrate the biologic effect of TNFRSF12A.
Product Category
 Recombinant Protein
Target
 TNFRSF12A/TWEAKR
Synonyms
 FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu28-Per80
Molecular Weight
 The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test).
Human TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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