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Human TNFRSF12A/TWEAKR Protein (TNF-HM20A) Summary
Product Description |
Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Glu28-Per80. |
Product Background |
Tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a) is a receptor for tumor necrosis factor superfamily member 12 (Tnfsf12). Tnfrsf12a is highly inducible and plays a key role in the development of cardiac hypertrophy and heart failure. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human TNFRSF12A/TWEAKR Protein (TNF-HM20A) Specifications/Details
Protein |
TNFRSF12A/TWEAKR |
Synonyms |
FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu28-Per80 |
Molecular Weight |
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TNFRSF12A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test). |
|
|
Human TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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