Human TNFSF12 Protein (TNF-HM212)
Catalog: TNF-HM212
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| Product Description | Recombinant Human TNFSF12 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Ser43-His249. |
| Product Category | Recombinant Protein |
| Target | TNFSF12 |
| Gene/Protein ID | O43508-1 |
| Species | Human |
| Exact Sequence | Ser43-His249 |
| Molecular Weight | The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 75.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22μm filtered solution in PBS, 200mM L-Arginine (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. |
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Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 75.5ng/ml determined by ELISA (QC Test). |
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The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC. |
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Human TNFSF12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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