Human TNFSF12/TWEAK Protein (TNF-HM212)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser43-His249

Catalog #TNF-HM212

Size

100UG

500UG

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Product Description	Recombinant Human TNFSF12/TWEAK Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser43-His249.
Product Background	Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
Product Category	Recombinant Protein / Antibody discovery

Protein	TNFSF12/TWEAK
Synonyms	APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
Accession	O43508-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser43-His249
Molecular Weight	The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
	Human TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC.