Product Description |
Recombinant Human TNFSF12/TWEAK Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser43-His249. |
Product Background |
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation. |
Product Category |
Recombinant Protein |
Target |
TNFSF12/TWEAK |
Synonyms |
APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser43-His249 |
Molecular Weight |
The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). |
|
|
Human TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC. |
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