Human TNFSF12 Protein (TNF-HM212)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 75.5ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser43-His249

Catalog: TNF-HM212

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Product Description Recombinant Human TNFSF12 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Ser43-His249.
Product Category Recombinant Protein
Target TNFSF12
Gene/Protein ID O43508-1
Species Human
Exact Sequence Ser43-His249
Molecular Weight The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 75.5ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS, 200mM L-Arginine (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 75.5ng/ml determined by ELISA.
The purity of Human TNFSF12 is greater than 95% as determined by SEC-HPLC.
Human TNFSF12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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