• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ser43-His249
Catalog #TNF-HM212
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Human TNFSF12/TWEAK Protein (TNF-HM212) Summary

Product Description

 Recombinant Human TNFSF12/TWEAK Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser43-His249.

Product Background

 Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human TNFSF12/TWEAK Protein (TNF-HM212) Specifications/Details

Protein
 TNFSF12/TWEAK
Synonyms
 APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser43-His249
Molecular Weight
 The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). Immobilized Human TNFSF12, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
Human TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC. The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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