Human TNFSF15 Protein (FSF-HM215)

Express System: HEK293
Product Tag: N-monomeric Fc
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Human TNFSF15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu72-Leu251

Catalog: FSF-HM215

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Product Description	Recombinant Human TNFSF15 Protein is expressed from HEK293 with monomeric Fc tag at the N-terminus.It contains Leu72-Leu251.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein

Target	TNFSF15
Gene/Protein ID	O95150-1
Species	Human
Exact Sequence	Leu72-Leu251
Molecular Weight	The protein has a predicted MW of 44.87 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TNFSF15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-monomeric Fc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human TNFSF15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA.
	Immobilized Mouse DR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFSF15, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA (QC Test).
	The purity of Human TNFSF15 is greater than 90% as determined by SEC-HPLC.
	Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.