Product Description |
Recombinant Human TNFSF15 Protein is expressed from HEK293 with monomeric Fc tag at the N-terminus.It contains Leu72-Leu251. |
Product Background |
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
Product Category |
Recombinant Protein |
Target |
TNFSF15 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu72-Leu251 |
Molecular Weight |
The protein has a predicted MW of 44.87 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TNFSF15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-monomeric Fc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human TNFSF15, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. |
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|
Immobilized Mouse DR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFSF15, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA (QC Test). |
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Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human TNFSF15 is greater than 90% as determined by SEC-HPLC. |
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